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RESEARCH ARTICLE
Year : 2017  |  Volume : 7  |  Issue : 1  |  Page : 28-36

A porcine ex vivo lung perfusion model with maximal argon exposure to attenuate ischemia-reperfusion injury

An Martens1, Sofie Ordies1, Bart M Vanaudenaerde2, Stijn E Verleden2, Robin Vos2, Geert M Verleden2, Eric K Verbeken3, Dirk E Van Raemdonck4, Sandra Claes5, Dominique Schols5, Matthieu Chalopin6, Ira Katz6, Geraldine Farjot6, Arne P Neyrinck M.D., Ph.D. 1
1 Laboratory of Anesthesiology and Algology, Department of Cardiovascular Sciences, Katholieke Universiteit Leuven and University Hospitals, Leuven; Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven, Belgium
2 Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven; Laboratory of Pneumology, Department of Clinical and Experimental Medicine, Lung Transplant Unit, Katholieke Universiteit Leuven and University Hospitals, Leuven, Belgium
3 University Hospitals Leuven, Department of Histopathology, Leuven, Belgium
4 Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven; Laboratory of Experimental Thoracic Surgery, Department of Clinical and Experimental Medicine, Katholieke Universiteit Leuven and University Hospitals, Leuven, Belgium
5 Laboratory of Virology and Chemotherapy (Rega Institute), Department of Microbiology and Immunology, Katholieke Universiteit Leuven, Leuven, Belgium
6 Air Liquide Santé International Medical R&D; Paris-Saclay Research Center, Jouy-en Josas, France

Correspondence Address:
Arne P Neyrinck
Laboratory of Anesthesiology and Algology, Department of Cardiovascular Sciences, Katholieke Universiteit Leuven and University Hospitals, Leuven; Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven
Belgium
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Source of Support: Air Liquide Santé International Medical R&D (Paris-Saclay, France), No. MEDGAS 13-360; Clinical Research Fund UZ Leuven (Leuven, Belgium)., Conflict of Interest: None


DOI: 10.4103/2045-9912.202907

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Argon (Ar) is a noble gas with known organoprotective effects in rodents and in vitro models. In a previous study we failed to find a postconditioning effect of Ar during ex vivo lung perfusion (EVLP) on warm-ischemic injury in a porcine model. In this study, we further investigated a prolonged exposure to Ar to decrease cold ischemia-reperfusion injury after lung transplantation in a porcine model with EVLP assessment. Domestic pigs (n = 6/group) were pre-conditioned for 6 hours with 21% O 2 and 79% N 2 (CONTR) or 79% Ar (ARG). Subsequently, lungs were cold flushed and stored inflated on ice for 18 hours inflated with the same gas mixtures. Next, lungs were perfused for 4 hours on EVLP (acellular) while ventilated with 12% O 2 and 88% N 2 (CONTR group) or 88% Ar (ARG group). The perfusate was saturated with the same gas mixture but with the addition of CO 2 to an end-tidal CO 2 of 35-45 mmHg. The saturated perfusate was drained and lungs were perfused with whole blood for an additional 2 hours on EVLP. Evaluation at the end of EVLP did not show significant effects on physiologic parameters by prolonged exposure to Ar. Also wet-to-dry weight ratio did not improve in the ARG group. Although in other organ systems protective effects of Ar have been shown, we did not detect beneficial effects of a high concentration of Ar on cold pulmonary ischemia-reperfusion injury in a porcine lung model after prolonged exposure to Ar in this porcine model with EVLP assessment.


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