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  Indian J Med Microbiol
 

Figure 4: General mode of operation of the chemiluminescence method for detection of NO. Note: (A) Working model of chemiluniescence method for NO detection. Adapted from Bates,[33] Hetrick and Schoenfisch.[34] (B) Luciferase-based chemiluminescence method for detection of NO concentration in cell cultures. Adapted from Woldman et al.[35] NO: Nitric oxide; PMT: photomultiplier; NO2*: nitrogen dioxide in the excited state; hν: luminescence (h: Planck’s constant, ν: frequency of photon); ONOO: highly oxidative peroxynitrite; GTP: guanosine triphosphate; cGMP: cyclic guanosine monophosphate; PPi: pyrophosphate; APS: adenosine-5′ phosphosulfate; ATP: adenosine triphosphate; AMP: adenosine monophosphate.

Figure 4: General mode of operation of the chemiluminescence method for detection of NO.
Note: (A) Working model of chemiluniescence method for NO detection. Adapted from Bates,<sup>[33]</sup> Hetrick and Schoenfisch.<sup>[34]</sup> (B) Luciferase-based chemiluminescence method for detection of NO concentration in cell cultures. Adapted from Woldman et al.<sup>[35]</sup> NO: Nitric oxide; PMT: photomultiplier; NO<sub>2</sub>*: nitrogen dioxide in the excited state; hν: luminescence (h: Planck’s constant, ν: frequency of photon); ONOO<sup>–</sup>: highly oxidative peroxynitrite; GTP: guanosine triphosphate; cGMP: cyclic guanosine monophosphate; PPi: pyrophosphate; APS: adenosine-5′ phosphosulfate; ATP: adenosine triphosphate; AMP: adenosine monophosphate.