Figure 2: Isolation, amplification and identification of tumor infiltrating lymphocyte (TIL) cells. Note: (A) After the isolation and amplification of TIL cells, the cell suspension was observed under the light microscope (IX37, Olympus, Tokyo, Japan). Scale bars: left: 200 μm, right: 20 μm. (B) CD3 flow cytometry detection to identify the TILs. (C) CD4 and CD8 classification of CD3+ TIL cells by flow cytometry. FITC: fluorescein isothiocyanate; PE: P-phycoerythrin.