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  Indian J Med Microbiol
 

Figure 3: The effect of hydrogen on mRNA and protein expression of inflammation related genes in brain at 24 hours after middle cerebral artery occlusion. Note: (A–D) mRNA expression: interleukin (IL)-1β (A), IL-6 (B), tumor necrosis factor (TNF)-α (C) and IL-10 (D) (real-time PCR); (E–H) protein expression: IL-1β (E), IL-6 (F), TNF-α (G) and IL-10 (H) (enzyme-linked immunosorbent assay). Data are expressed as the mean ± SEM (n = 4–5 per group); *P < 0.05, **P < 0.01, ***P < 0.001, vs. sham group; #P < 0.05, ##P < 0.01, ###P < 0.001, vs. I/R group (one-way analysis of variance followed by Bonferroni’s multiple comparison test). I/R: Ischemia/reperfusion.

Figure 3: The effect of hydrogen on mRNA and protein expression of inflammation related genes in brain at 24 hours after middle cerebral artery occlusion.
Note: (A–D) mRNA expression: interleukin (IL)-1β (A), IL-6 (B), tumor necrosis factor (TNF)-α (C) and IL-10 (D) (real-time PCR); (E–H) protein expression: IL-1β (E), IL-6 (F), TNF-α (G) and IL-10 (H) (enzyme-linked immunosorbent assay). Data are expressed as the mean ± SEM (<i>n</i> = 4–5 per group); *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001, <i>vs</i>. sham group; #<i>P</i> < 0.05, ##<i>P</i> < 0.01, ###<i>P</i> < 0.001, <i>vs</i>. I/R group (one-way analysis of variance followed by Bonferroni’s multiple comparison test). I/R: Ischemia/reperfusion.