Figure 2: The effect of hydrogen on number and morphological change of microglia in brain at 24 hours after ischemic stroke. Note: (A) The morphological change of microglia in ischemic brain tissues (immunofluorescence staining). Blue: DAPI; Red: tetramethylrhodamine isothiocyanate (TRATC). Scale bars: 200 µm. (B) Photomicrographs showing examples of microglia classified into three different phenotypes: ramified, intermediate and amoeboid/round. Scale bars: 20 µm. (C) Proportion of microglia with different morphological phenotypes of Iba-1+ cells. (D) Quantitative result of total number of microglia (Iba-1+ cells). Dara are expressed as the mean ± SEM of three brain sections. *P < 0.05, **P < 0.01, ***P < 0.001, vs. sham group; ##P < 0.01, ###P < 0.001, vs. I/R group (one-way analysis of variance followed by Bonferroni’s multiple comparison test). I/R: Ischemia/reperfusion.