Figure 1: Hydrogen reduced infarct volume, alleviated brain edema and improved neurobehavioral deficits at 24 hours after middle cerebral artery occlusion in mice. Note: (A) 2,3,5-Triphenyltetrazolium chloride staining (representative sections). (B) Quantification of 2,3,5-triphenyltetrazolium chloride staining (n = 5–7/group). (C) Quantitative result of postoperative survival rate (n = 20 per group). (D) Evaluation of brain edema development (n = 4–5 per group). (E) Neurological deficit score (n = 16–20 per group). Dara are presented as the mean ± SEM. **P < 0.01, ***P < 0.001, vs. sham group; #P < 0.05, ###P < 0.001, vs. I/R group (one-way analysis of variance followed by Bonferroni’s multiple comparison test). I/R: Ischemia/reperfusion.