Figure 3: Effect of hydrogen-rich water (HRW) on the stress protein expression in the placenta of placental stress rats induced by water restriction (western blot assay). Note: The expression of placental stress proteins reached the highest level in water restriction group (WR). While the expression level in hydrogen-rich water group (HW) was usually higher than that in control group (CG), and lower than that in WR. (A) Placental protein expression in different groups. (B) Relative expression of the target protein in the placentas. β-Actin was used an internal control. Data are shown as the mean ± SEM, and analyzed by repeated measures analysis of variance. *P < 0.05, vs. CG group; #P < 0.05, vs. WR group. AT1R: Angiotensin II type 1 receptor; NFκB: nuclear factor κB; MDA: malondialdehyde; JUK: c-Jun N-terminal kinase.