Close
  Indian J Med Microbiol
 

Figure 4: Representative images showing the influence of high fractional oxygen on the secretion of angiogenesis factors from MDA-MB-231 (A) and MCF-7 (B) breast adenocarcinoma cells. Note: Cells were exposed to medical air, 30%, 60% or 80% O2 for 3 hours as described in Materials and Methods, following which they were returned to the incubator for 24 hours. Conditioned medium was collected and applied to a commercially available membrane-based angiogenesis array and expression of secreted angiogenesis factors was detected as per the manufacturer’s protocol. (A) In comparison to medical air, increased secretion of regulated on activation normal T cell expressed (RANTES) (blue), monocyte chemotactic protein 1 (MCP-1) (pink), and vascular endothelial growth factor (VEGF) (orange) angiogenesis factors were detected in medium from MDA-MB-231 cells exposed to high fractional oxygen. (B) In comparison to medical air, increased secretion of angiogenin (red), interleukin-8 (green), and VEGF (orange) was detected in medium from MCF-7 cells exposed to high fractional oxygen. Semi-quantitative scanning densitometry confirmed these observations [Table 1]

Figure 4: Representative images showing the influence of high fractional oxygen on the secretion of angiogenesis factors from MDA-MB-231 (A) and MCF-7 (B) breast adenocarcinoma cells.
Note: Cells were exposed to medical air, 30%, 60% or 80% O<sub>2</sub> for 3 hours as described in Materials and Methods, following which they were returned to the incubator for 24 hours. Conditioned medium was collected and applied to a commercially available membrane-based angiogenesis array and expression of secreted angiogenesis factors was detected as per the manufacturer’s protocol. (A) In comparison to medical air, increased secretion of regulated on activation normal T cell expressed (RANTES) (blue), monocyte chemotactic protein 1 (MCP-1) (pink), and vascular endothelial growth factor (VEGF) (orange) angiogenesis factors were detected in medium from MDA-MB-231 cells exposed to high fractional oxygen. (B) In comparison to medical air, increased secretion of angiogenin (red), interleukin-8 (green), and VEGF (orange) was detected in medium from MCF-7 cells exposed to high fractional oxygen. Semi-quantitative scanning densitometry confirmed these observations [Table 1]