Figure 3: Reversal of oxygen-mediated effects on MDA-MB-231 and MCF-7 cell migration by dimethyloxaloylglycine (DMOG). Note: (A) Cells were incubated with either DMOG (1 mM) or a vehicle control (water) and the whole plate was exposed to either 60% O2 or control gas for 3 hours, following which migration was measured at 24 hours as described above. DMOG reduced migration of both cell lines in control gas and also reduced migration of MCF-7 cells in 60% O2. (B) Reduced migration did not reflect decreased cell density in the DMOG-exposed wells, as solubilisation of Coomassie blue-stained cells in plates used for migration studies did not reveal any difference in absorbance at 570 nm (n = 3). Values shown are mean (SEM) versus values for matched cells exposed to vehicle control at the same time *P ≤ 0.05, vs. vehicle control.