Figure 4: Tissue histopathology at 14 days post repetitive mild juvenile t raumatic brain injury (rmjTBI). Note: There was increased glial fibrillary acidic protein (GFAP) staining intensity (A) and ionized calcium binding adaptor molecule 1 (IBA1) staining intensity (B), indicating gliosis of astrocyte and microglia following rmjTBI. Hyperbaric oxygen (HBO) treatment alleviated the astrocyte hypertrophy and microglial activation. Scale bars: 10 μm. Data are presented as the mean ± SEM. *P < 0.05, vs. sham group, #P < 0.05, vs. rmjTBI + HBO group (one-way analysis of variance followed by Student-Newman-Keuls post hoc test).