Medical Gas Research

RESEARCH ARTICLE
Year
: 2018  |  Volume : 8  |  Issue : 4  |  Page : 154--159

Effects of hydrogen on polarization of macrophages and microglia in a stroke model


Ke Ning1, Wen-Wu Liu2, Jun-Long Huang1, Hong-Tao Lu1, Xue-Jun Sun1 
1 Department of Navy Aeromedicine, Faculty of Naval Medicine, Navy Medical University, Shanghai, China
2 Department of Diving and Hyperbaric Medicine, Faculty of Naval Medicine, Navy Medical University, Shanghai, China

Correspondence Address:
Xue-Jun Sun
Department of Navy Aeromedicine, Faculty of Naval Medicine, Navy Medical University, Shanghai
China

It has been confirmed that inflammation plays an important role in the pathogenesis of ischemic stroke. The polarization of microglia as an important participant in the inflammation following stroke is also found to be involved in stroke. This study aimed to investigate the effects of hydrogen gas on the polarization of macrophages/microglia in vitro. Raw264.7 cells were treated with lipopolysaccharides and then exposed to hydrogen. The microglia were treated with the supernatant from oxygen and glucose deprivation-treated neurons and then exposed to hydrogen. The phenotypes of Raw 264.7 cells and microglia were determined by flow cytometry, and cell morphology was observed. Results showed lipopolysaccharides significantly increased the M1 macrophages, and the supernatant from oxygen and glucose deprivation-treated neurons dramatically elevated the proportion of M1 microglia, but both treatments had little influence on the M2 cells. In addition, hydrogen treatment significantly inhibited the increase in M1 cells, but had no influence on M2 ones. Our findings suggest that the neuroprotection of hydrogen may be related to its regulation of microglia in the nervous system after stroke.


How to cite this article:
Ning K, Liu WW, Huang JL, Lu HT, Sun XJ. Effects of hydrogen on polarization of macrophages and microglia in a stroke model.Med Gas Res 2018;8:154-159


How to cite this URL:
Ning K, Liu WW, Huang JL, Lu HT, Sun XJ. Effects of hydrogen on polarization of macrophages and microglia in a stroke model. Med Gas Res [serial online] 2018 [cited 2019 Mar 26 ];8:154-159
Available from: http://www.medgasres.com/article.asp?issn=2045-9912;year=2018;volume=8;issue=4;spage=154;epage=159;aulast=Ning;type=0