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RESEARCH ARTICLE
Year : 2018  |  Volume : 8  |  Issue : 2  |  Page : 57-63

Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes


1 Department of Radiological Technology, Faculty of Health Sciences, Butsuryo College of Osaka, Osaka, Japan
2 Division of Biology, Faculty of Liberal Arts and Sciences, and Division of Quantum Radiation, Faculty of Technology, Osaka Prefecture University, Osaka, Japan
3 Prefectural University of Hiroshima; Japanese Center for AntiAging MedSciences, Hiroshima, Japan

Correspondence Address:
Yoshiharu Tanaka
Division of Biology, Faculty of Liberal Arts and Sciences, and Division of Quantum Radiation, Faculty of Technology, Osaka Prefecture University, Osaka
Japan
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Source of Support: This work was supported by a Grant-in-Aid for Scientific Research (KAKENHI No. 26350681 to QL) from the Ministry of Education, Culture, Sports, Science and Technology of Japan., Conflict of Interest: None


DOI: 10.4103/2045-9912.235128

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Many conventional studies on molecular hydrogen have not examined cell migration ability and the relationship between apoptosis and the cytoskeleton. Here we investigated the influence of hydrogen-occluding silica microparticles (H2-silica) on cell migration motility and changes of the cytoskeleton (F-actin) in normal human esophageal epithelial cells (HEEpiCs). As the results, cell migration was promoted, and formation of microvilli was activated in the 100 ppm (low concentration) scratched group. After performing a wound healing assay, cells exhibited migration after 48 hours and 72 hours for both 10 ppm and 100 ppm groups, suggesting that the wound-repairing effects could be attributed to the antioxidant ability of H2-silica. In scratched groups, high levels of activated caspase-3 were relatively expressed and presented a tendency to increase the observed Bax/Bcl-2 ratio at more than 300 ppm groups. The above-mentioned results show that H2-silica induced apoptosis in HEEpiCs, especially in the scratched cells. Toxicity may cause an exaggerated apoptosis. Furthermore, since the ratio of fascin/tubulin in the 100, 300, and 600 ppm groups tended to increase in both the scratched and the non-scratched control groups, H2-silica was thought to be able to promote fascin action on normal cells and may be have a proliferative effect.


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